Plasmid

Part:BBa_J100288:Design

Designed by: Hartlee Johnston   Group: Campbell M Lab   (2016-07-11)


pJC173b with gIII neg


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2481
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
    Illegal AgeI site found at 3407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5247
    Illegal SapI site found at 2648
    Illegal SapI site found at 3926


Design Notes

Used BbsI as a restriction enzyme because of the internal BsaI site.


Source

Part was designed using BbsI and GGA to remove a 210 bp section from J100265.

References